EDRN Reference Set Call

Wednesday, April 2, 2025

Present (Attendees at meeting in BOLD):

NCI:  Guillermo Marquez, Richard Mazurchuk, Indu Kohaar, Christos Patriotis, Amanda Skarlupka, Matt Young

DMCC: Ziding Feng, Jackie Dahlgren, Royce Malnik, Yingye Zheng

GI:

• Colon: MD Anderson: Robert Bresalier (Chair)
• Pancreas: University of Texas MD Anderson: Anirban Maitra
• University of Minnesota: Aasma Shaukat

Lung:

• Vanderbilt: Stephen Deppen (Co-chair)
• Boston University: Marc Lenburg
• Meso Scale Diagnostics: Martin Stengelin
• University of California, Los Angeles: Tran Linh
• University of Maryland: Sandy Stass

Prostate:

• Pacific Northwest National Laboratory: Karin Rodland
• Eastern Virginia Medical School: John Semmes
• Johns Hopkins: Hui Zhang
• University of Michigan: Javed Siddiqui

Breast/GYN:

• Arizona State University: Karen Anderson
• Massachusetts General Hospital: Steven Skates
• Johns Hopkins: Zhen Zhang
• Meso Scale Diagnostics: Martin Stengelin

BRL (All Groups):

• NIST: Hua-Jun He

Current Action Items:

1. DONE: Chris Li’s Application: Robert Bresalier will follow up with Steve Skates for his review.
2. GLNE 010: Matt Young will send Robert Bresalier the contact at U Maryland to discuss this further.
3. Yingye Zheng to meet with Statistical Working Group to discuss specimen sets being used for discovery.
4. Matt Young will locate the list of GLNE 010 Stool/DNA that Sandy Stass previously sent and re-distribute to the DMCC and Bob Bresalier. Update: this involved specimens that were sent to Dr. Stass’s lab to test a biomarker for Sandy Markowitz but nothing was done.  Dr. Stass wants to dispose of the specimens, but Robert Bresalier knows someone who would like the specimens, but he wants to know what these specimens are before sending them. Dr. Bresalier will follow up with Matt Young about this.
5. GLNE 010: Robert Bresalier will check with Ziding Feng about the composition of the specimens and deal with unblinding since the specimens are not being used for the study.
6. Bob Bresalier will follow-up with Xianghong (Jasmine) Zhou’s regarding her email about whether 1ml will work for their DCP Rapid 50/50 request:  Robert Bresalier stated that Dr. Zhou is tweaking their assay so that the volume of specimen needed is less because more than 1 mL is too much. Dr. Bresalier will follow up with her. 

Discussion:

Review of Applications: 

• Chris Li/BRS: Robert Bresalier has not received the responses from the 3 reviewers yet.  He will follow up with Steve Skates for his review. 
• Amit Singal: The application will be reviewed by Ziding Feng and the PIs at the next HEDS PI call.  The DMCC has all the data and the DMCC will perform the analysis. If Ziding Feng’s review is consistent with the group’s comments, then it can be approved. 

Comprehensive List of Reference Sets: Robert Bresalier wants a comprehensive list of all the reference sets available so that it is searchable and accessible. There are no delays in getting specimens to applicants and aliquoting specimens. There does seem to be a delay in processing specimens.  FNLCR is still able to send labels. Steve Skates is sending the Uterine Lavage to another lab.

GLNE 010: There are 3 freezers full of stool samples. Robert Bresalier has a home for these specimens, but FNLCR will have to pay for shipping them to a new site. There is a company that has a high-throughput microbiome platform and an algorithm. Issues with blinding will need to be addressed further between Matt Young, Robert Bresalier and Sudhir Srivastava. Matt Young will send Robert Bresalier the contact at U Maryland to discuss this further. 

Scientific Workshop: This is still going to happen. Attendees will need to re-book their hotel rooms. 

Next Call: Wednesday, May 7th at 8 a.m. Pacific/11 a.m. Eastern.